File:Stem cells neurospheres drived from Huntingtons Disease hippocampus.png

Large stem cell-derived neurospheres were generated from 33-week old HD hippocampus, but not WT hippocampus.

(A) Similar numbers of large (≥250 µm in diameter) hippocampal neurospheres were generated from HD (n = 4) and WT (n = 4) mice at 5 weeks of age. However, by 33 weeks, large neurospheres could only be generated from the HD hippocampus (n = 4) and not the WT (n = 5) hippocampus. Data are expressed as mean ± s.e.m. Representative images of a small progenitor-derived neurosphere from a 33-week old WT hippocampus (B) and a large stem cell-derived neurosphere from a 33-week old HD hippocampus (C). Small neurospheres generated from the 33-week old WT hippocampus gave rise exclusively to astrocytes (shown in green; D), whereas the large neurospheres generated from the 33-week old HD hippocampus gave rise to astrocytes (green) and a small number of neurons (red; E). Scale bars in B and C are 100 µm.

Copyright: © 2011 Walker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018153