File:Liver hepatocyte from stem cell.png

Overview of liver embryogenesis on which liver differentiation protocol is based.

Genes specifically expressed at different steps during liver development are shown, in italics, under each step of lineage commitment. Cytokines used as well as other culture conditions are shown.

"The protocol supports the stepwise differentiation of human embryonic stem cells (ESC) to cells with characteristics of primitive streak (PS)/mesendoderm (ME)/definitive endoderm (DE), hepatoblasts, and finally cells with phenotypic and functional characteristics of hepatocytes. Remarkably, the same protocol can also differentiate rat multipotent adult progenitor cells (rMAPCs) to hepatocyte-like cells, even though rMAPC are isolated clonally from cultured rat bone marrow (BM) and have characteristics of primitive endoderm cells."

Original File Name: Figure 1. Journal.pone.0012101.g001.png http://www.plosone.org/article/slideshow.action?uri=info:doi/10.1371/journal.pone.0012101&imageURI=info:doi/10.1371/journal.pone.0012101.g001

Reference

<pubmed>20711405</pubmed>| PLoS One.

Copyright: © 2010 Roelandt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.