File:Canonical Wnt signaling in Wnt5a KO limb buds.jpg
Original file (1,280 × 701 pixels, file size: 132 KB, MIME type: image/jpeg)
Canonical Wnt signaling was increased in the Wnt-5a-/- limb buds.
Gene expression in embryos was examined by whole mount in situ hybridization.
(A) At 12.5 dpc, the expression of Sox9, which marks early chondrogenesis, was not detected in the distal-most limb bud of the Wnt-5a-/- limb (arrow).
(B) Ectopic LacZ staining (arrow) was detected in the Wnt-5a-/-/TOPGAL distal limb at 12.5 dpc. (C) The ß-catenin protein level was increased in the distal Wnt-5a-/- limb at 11.5 dpc. (D) Sfrp-2 expressing cells (red), but not control cells (blue), induced ectopic expression of ColII (arrows), a marker for chondrocyte differentiation in both wild-type and Wnt-5a-/- limbs.
Original File name: 200303158f5.jpeg
Figure 5. http://jcb.rupress.org/content/vol162/issue5/images/large/200303158f5.jpeg
Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent beta-catenin degradation. Topol L, Jiang X, Choi H, Garrett-Beal L, Carolan PJ, Yang Y. J Cell Biol. 2003 Sep 1;162(5):899-908. PMID: 12952940
Copyright
Rockefeller University Press - Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/ ). (More? Help:Copyright Tutorial)
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 15:38, 22 March 2010 | 1,280 × 701 (132 KB) | S8600021 (talk | contribs) | Canonical Wnt signaling was increased in the Wnt-5a-/- limb buds. Gene expression in embryos was examined by whole mount in situ hybridization. (A) At 12.5 dpc, the expression of Sox9, which marks early chondrogenesis, was not detected in the distal-m |
You cannot overwrite this file.
File usage
The following page uses this file: