Category:Super Resolution Microscopy

This Embryology category lists content related to the imaging technique of super resolution microscopy.

Super-resolution techniques break the diffraction limit by temporally or spatially modulating the excitation or activation light.

Any microscopy technique that overcomes the resolution limit of conventional light microscopy by at least a factor of two is considered to be a super-resolution technique. The resolution limit of conventional light microscopy is ~250 nm in the x and y direction, and >450–700 nm in the z direction.

Point-spread function (PSF)

• minimum-size point source or object that can be resolved by a microscope.
• resolution limit is the fixed size of the spread of a single point of light that is diffracted through a microscope.
• Rayleigh (R) criterion - R=0.61λ/NA, where NA is the numerical aperture.

References

<pubmed>21536831 </pubmed>