|from Embryology (16 Apr 2014)||Translate/Bookmark|
The mouse (taxon-mus) has always been a good embryological model, generating easily (litters 8-20) and quickly (21d). Mouse embryology really expanded when molecular biologists used mice for gene knockouts. Suddenly it was necessary to understand development in order to understand the effect of knocking out the gene.
There are over 450 different strains of inbred research mice, and these strains have recently been organized into a chart. Those interested in the mouse reproductive cycle should also look at the mouse estrous cycle.
There are several systems for staging mouse development. The original and most widely used is the Theiler Stages system, which divides mouse development into 26 prenatal and 2 postnatal stages. 
- Mouse Links: Introduction | Mouse Stages | Mouse Timeline | Mouse Timeline Detailed | Mouse Estrous Cycle | Mouse Knockout | Movie - Cephalic Plexus | ANAT2341 Project (2009) | Category:Mouse
- Mouse Stages: E1 | E2.5 | E3 | E3.5 | E4.5 | E7.5 | E8.5 | E9.0 | E9.5 | E10 | E10.5 | E11 | E11.5 | E12 | E12.5 | E13 | E13.5 | E14 | E14.5 | E15 | E15.5 | E16 | E16.5 | E17 | E18 | E18.5 | E19 | E20 | Timeline | About timed pregnancy
- Note that these Carnegie stages are only approximate day timings for average of embryos.
- Links: Carnegie Stage Comparison
Some Recent Findings
|More recent papers|
References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.
Nina Mirderikvand, Baharak Mohammadzadeh Asl, Parvaneh Naserzadeh, Fatemeh Shaki, Mohammad Shokrzadeh, Jalal Pourahmad Embryo toxic effects of depleted uranium on the morphology of the mouse fetus. Iran J Pharm Res: 2014, 13(1);199-206 PMID:24734072 Sergio Arroyo, Corbett Bennett, Shaul Hestrin Nicotinic modulation of cortical circuits. Front Neural Circuits: 2014, 8();30 PMID:24734005 Tomonori Furukawa, Junko Yamada, Tenpei Akita, Yoshitaka Matsushima, Yuchio Yanagawa, Atsuo Fukuda Roles of taurine-mediated tonic GABAA receptor activation in the radial migration of neurons in the fetal mouse cerebral cortex. Front Cell Neurosci: 2014, 8();88 PMID:24734001 Helen Connaris, Elena A Govorkova, Yvonne Ligertwood, Bernadette M Dutia, Lei Yang, Sandra Tauber, Margaret A Taylor, Nadiawati Alias, Robert Hagan, Anthony A Nash, Robert G Webster, Garry L Taylor Prevention of influenza by targeting host receptors using engineered proteins. Proc. Natl. Acad. Sci. U.S.A.: 2014; PMID:24733924 Xin Ma, Zhen Chen, Dong Hua, Dongxu He, Linjun Wang, Peng Zhang, Junqi Wang, Yanfei Cai, Caiji Gao, Xiaodong Zhang, Fangfang Zhang, Teng Wang, Tingting Hong, Linfang Jin, Xiaowei Qi, Shuxian Chen, Xiaoting Gu, Dangtong Yang, Qiongxi Pan, Yifei Zhu, Yun Chen, Daozhen Chen, Liwen Jiang, Xiaofeng Han, Yanyun Zhang, Jian Jin, Xiaoqiang Yao Essential role for TrpC5-containing extracellular vesicles in breast cancer with chemotherapeutic resistance. Proc. Natl. Acad. Sci. U.S.A.: 2014; PMID:24733904
|Postnatal Animal Models||Mouse||Rat||Pig|
|Pregnancy period (days)||18 – 21||21 – 23||110 – 118|
|Placenta type|| Discoidal, decidual
| Discoidal, decidual
|Litter size||6 – 12||6 – 15||11 – 16|
|Birth weight (g)||0.5 – 1.5||3 – 5||900 – 1600|
|Weaning weight male/female (g)||18 – 25/16 – 25||55 – 90/45 – 80||6000 – 8000|
|Suckling period (days)||21–28||21||28–49|
|Solid diet beginning (days)||10||12||12 – 15|
|Puberty male/female (week)||4 – 6/5||6/6 – 8||20 – 28|
|Life expectancy (years)||1 - 2||2 - 3||14 – 18|
Early Mouse Development
|zygote||blastomeres||morula||early blastocyst||hatched blastocyst|
Early mouse development model
| Live cell imaging and tracking
|| Simulation of Zygote to Blastocyst
- Links: Mouse Stages
Later Mouse Development
- Links: Limb Development
Carnegie Stages Comparison
The table below gives an approximate comparison of human, mouse and rat embryos based upon Carnegie staging.
- Note that these Carnegie stages are only approximate day timings for average of embryos.
- Links: Carnegie Stage Comparison
- placenta originates from the ectoplacental cone and the extra-embryonic ectoderm.
- endothelial cells derive from the allantois.
- embryonic day (E 10) - placenta divided into three layers associated with maternal decidual cells.
- Labyrinth - (equivalent to human villi) a selective barrier on the fetal side, is an array of fetal and maternal vessels.
- Junctional zone - (spongy layer) produce hormones and contains numerous cavities. The trophoblast cells form spongiotrophoblasts and the glycogen cells, that later (E 12.5) migrate into the maternal decidua.
- Giant cells - next to the uterine cells form the outermost fetal cell layer until (E 12).
|Mouse Placenta Vasculature (E16.5)|
|superior view (maternal side)||lateral view (maternal side at bottom)|
The process of spermatogenesis takes approximately 35 days:
- mitotic phase (11 days)
- meiotic phase (10 days)
- post-meiotic phase (14 days)
Spermatogonial stem cells (SSCs)
The diploid germ cells, spermatogonial stem cells (SSCs), are located on the basement membrane of the seminiferous tubules
- adult mouse testis about 30,000 SSCs
- either divides into two new single cells
- or into a pair of spermatogonia (Apr)
- that do not complete cytokinesis and stay connected by an intercellular bridge
Primitive spermatogonia subset
- Asingle (As, single isolated spermatogonia)
- Apaired (Apr, interconnected spermatogonial pairs)
- Aaligned (Aal, interconnected 4, 8, or 16 spermatogonia)
- specifically termed Aal-4, Aal-8, and Aal-16
Primitive spermatogonia cells transform without cell division into more differentiating A1 spermatogonia that undergo 6 mitotic and 2 meiotic divisions to eventually form haploid spermatids.
Mouse limb skeleton cartoon
Fore-limb and hind-limb buds for stages E9.5 to E13.5. Hindlimbs are morphologically delayed by about half a day.
- Light blue - indicate mesenchymal condensations.
- Thick black lines - indicate cartilage as determined by alcian blue staining.
Change in cell types and tissue formation as a function of mouse developmental stage.
- Links: Limb Development
The data below is summarised from a study of early neural development in the mouse.
- initial fusion of apposing neural folds occurred at the level of the intermediate point between the third and fourth somites (caudal myelencephalon) both rostrally and caudally
- second fusion - at the original rostral end of the neural plate (rostrodorsally).
- third fusion - in the caudal diencephalon (rostrally and caudally)
- followed by complete closure of the telencephalic neuropore at the midpoint of the telencephalic roof
- then complete closure of the metencephalic neuropore at the rostral part of the metencephalic roof
- fourth fusion - at the original caudal end of the neural plate (rostrally)
- caudal neuropore completely closed at the level of the future 33rd somite
A high-resolution description of the developing murine genitourinary tract from Theiler stage (TS) 17 (E10.5) through to TS27 (E19.5) and then to postnatal day 3 was published in 2007.
The GenitoUrinary Development Molecular Anatomy Project (GUDMAP) is a consortium of laboratories working to provide the scientific and medical community with tools to facilitate research. They intend to develop:
- a molecular atlas of gene expression for the developing organs of the GenitoUrinary (GU) tract
- a high resolution molecular anatomy that highlights development of the GU system
- mouse strains to facilitate developmental and functional studies within the GU system
- tutorials describing GU organogenesis
- rapid access to primary data via the GUDMAP database
Vertebrate lung development is generally divided into 5 stages, based upon growth and histological appearance. Mouse age data
|Embryonic||E9 to E11.5||lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs|
|Pseudoglandular||E11.5 to E16.5||conducting epithelial tubes surrounded by thick mesenchyme are formed, extensive airway branching|
|Canalicular||E16.5 to E17.5||bronchioles are produced, increasing number of capillaries in close contact with cuboidal epithelium and the beginning of alveolar epithelium development|
|Saccular||E17.5 to PN5||alveolar ducts and air sacs are developed|
|Alveolar||PN5 to PN28||secondary septation occurs, marked increase of the number and size of capillaries and alveoli|
Human and Mouse Comparison
|Embryonic||week 4 to 5||E9 to E11.5||lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs|
|Pseudoglandular||week 5 to 17||E11.5 to E16.5||conducting epithelial tubes surrounded by thick mesenchyme are formed, extensive airway branching|
|Canalicular||week 16 to 25||E16.5 to E17.5||bronchioles are produced, increasing number of capillaries in close contact with cuboidal epithelium and the beginning of alveolar epithelium development|
|Saccular||week 24 to 40||E17.5 to PN5||alveolar ducts and air sacs are developed|
|Alveolar||late fetal to 8 years||PN5 to PN28||secondary septation occurs, marked increase of the number and size of capillaries and alveoli|
Two postnatal phases identified:
- first phase - (pnd 1 (birth) to pnd 12) corresponds to the hypo-responsive period (SHRP) in the rat. Basal corticosterone levels were low and novelty exposure did not enhance corticosterone or ACTH levels. High expression of CRH in the paraventricular nucleus (PVN) of the hypothalamus. Expression levels of GR in the hippocampus and UCN3 in the perifornical area are low at birth but increase significantly during the SHRP, both reach maximum expression level at pnd 12.
- second phase - mice developed past the SHRP exhibit enhanced corticosterone basal levels and a response of ACTH and corticosterone to mild novelty stress. CRH expression was decreased significantly, expression of urocortin 3 (UCN3) and glucocorticoid receptor (GR) remained high, with a small decrease at pnd 16.
Knowledge about mouse development has rapidly expanded as it has become the model animal system for genetic "knock out " studies. This technology actually requires development of defined breeding programs, pseudo-pregnancy, in vitro fertilization, molecular biology, and good old fashioned histology. Without understanding normal development the molecular biologists don't stand a hope of understanding what their gene knock out has done. There is a database of all existing mouse knockouts and their consequences.
Murine Development Control Genes
Kessel, M. and Gruss, P. Science 249 374-379 (1990)
An early review of the genes, and method of identifying them, involved in early mouse development. In particular discusses Homeobox genes. (homeobox is 183bp encoding a 61 amino acid DNA-binding domain)
- Gene families
The Genealogy Chart of Inbred Strains
This Nature paper chart shows the origins and relationships of inbred mouse strains. The chart is available as a PDF document [Media:Mouse_genealogy.pdf Locally] or from JAX Labs and was originally published by Beck etal., 2000.
Mouse Genome completed December 2002, a draft sequence and analysis of the genome of the C57BL/6J mouse strain.
- less than 30,000 genes
- estimated size is 2.5 Gb, smaller than the human genome
- about 40% of the human and mouse genomes can be directly aligned
- about 80% of human genes have one corresponding gene in the mouse genome
- Links: Mouse Genome Sequencing: Mus musculus | Mouse Genome Informatics | Mouse Genome Project | Nature - Mouse Genome
- ↑ 1.0 1.1 Graciana Diez-Roux, Sandro Banfi, Marc Sultan, Lars Geffers, Santosh Anand, David Rozado, Alon Magen, Elena Canidio, Massimiliano Pagani, Ivana Peluso, Nathalie Lin-Marq, Muriel Koch, Marchesa Bilio, Immacolata Cantiello, Roberta Verde, Cristian De Masi, Salvatore A Bianchi, Juliette Cicchini, Elodie Perroud, Shprese Mehmeti, Emilie Dagand, Sabine Schrinner, Asja Nürnberger, Katja Schmidt, Katja Metz, Christina Zwingmann, Norbert Brieske, Cindy Springer, Ana Martinez Hernandez, Sarah Herzog, Frauke Grabbe, Cornelia Sieverding, Barbara Fischer, Kathrin Schrader, Maren Brockmeyer, Sarah Dettmer, Christin Helbig, Violaine Alunni, Marie-Annick Battaini, Carole Mura, Charlotte N Henrichsen, Raquel Garcia-Lopez, Diego Echevarria, Eduardo Puelles, Elena Garcia-Calero, Stefan Kruse, Markus Uhr, Christine Kauck, Guangjie Feng, Nestor Milyaev, Chuang Kee Ong, Lalit Kumar, MeiSze Lam, Colin A Semple, Attila Gyenesei, Stefan Mundlos, Uwe Radelof, Hans Lehrach, Paolo Sarmientos, Alexandre Reymond, Duncan R Davidson, Pascal Dollé, Stylianos E Antonarakis, Marie-Laure Yaspo, Salvador Martinez, Richard A Baldock, Gregor Eichele, Andrea Ballabio A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol.: 2011, 9(1);e1000582 PMID:21267068 | PLoS Biol. | Eurexpress transcriptome atlas
- ↑ Yoshiaki V Nishimura, Tomoyasu Shinoda, Yutaka Inaguma, Hidenori Ito, Koh-Ichi Nagata Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development. Med Mol Morphol: 2012, 45(1);1-6 PMID:22431177
- ↑ Katsuyoshi Takaoka, Hiroshi Hamada Cell fate decisions and axis determination in the early mouse embryo. Development: 2012, 139(1);3-14 PMID:22147950
- ↑ William C Skarnes, Barry Rosen, Anthony P West, Manousos Koutsourakis, Wendy Bushell, Vivek Iyer, Alejandro O Mujica, Mark Thomas, Jennifer Harrow, Tony Cox, David Jackson, Jessica Severin, Patrick Biggs, Jun Fu, Michael Nefedov, Pieter J de Jong, A Francis Stewart, Allan Bradley A conditional knockout resource for the genome-wide study of mouse gene function. Nature: 2011, 474(7351);337-42 PMID:21677750
- ↑ Francisco J Pérez-Cano, Àngels Franch, Cristina Castellote, Margarida Castell The suckling rat as a model for immunonutrition studies in early life. Clin. Dev. Immunol.: 2012, 2012();537310 PMID:22899949 | PMC3415261 | Clin Dev Immunol.
- ↑ 6.0 6.1 Pawel Krupinski, Vijay Chickarmane, Carsten Peterson Simulating the mammalian blastocyst--molecular and mechanical interactions pattern the embryo. PLoS Comput. Biol.: 2011, 7(5);e1001128 PMID:21573197 | PMC3088645 | PLoS Comput Biol.
- ↑ Samantha A Morris, Roy T Y Teo, Huiliang Li, Paul Robson, David M Glover, Magdalena Zernicka-Goetz Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo. Proc. Natl. Acad. Sci. U.S.A.: 2010, 107(14);6364-9 PMID:20308546 | PMC2852013 | PNAS
- ↑ Zhou Q, Griswold MD. Regulation of spermatogonia. StemBook [Internet]. Cambridge (MA): Harvard Stem Cell Institute. PMID20614596 | http://www.ncbi.nlm.nih.gov/books/NBK27035/ Bookshelf]
- ↑ 9.0 9.1 Leila Taher, Nicole M Collette, Deepa Murugesh, Evan Maxwell, Ivan Ovcharenko, Gabriela G Loots Global gene expression analysis of murine limb development. PLoS ONE: 2011, 6(12);e28358 PMID:22174793
- ↑ Neurulation in the mouse: manner and timing of neural tube closure. Sakai Y. Anat Rec. 1989 Feb;223(2):194-203. PMID: 2712345
- ↑ The histogenetic potential of neural plate cells of early-somite-stage mouse embryos. Chan WY, Tam PP. J Embryol Exp Morphol. 1986 Jul;96:183-93. PMID: 3805982
- ↑ Neurulation in the mouse. I. The ontogenesis of neural segments and the determination of topographical regions in a central nervous system. Sakai Y. Anat Rec. 1987 Aug;218(4):450-7. PMID: 3662046
- ↑ Melissa H Little, Jane Brennan, Kylie Georgas, Jamie A Davies, Duncan R Davidson, Richard A Baldock, Annemiek Beverdam, John F Bertram, Blanche Capel, Han Sheng Chiu, Dave Clements, Luise Cullen-McEwen, Jean Fleming, Thierry Gilbert, Doris Herzlinger, Derek Houghton, Matt H Kaufman, Elena Kleymenova, Peter A Koopman, Alfor G Lewis, Andrew P McMahon, Cathy L Mendelsohn, Eleanor K Mitchell, Bree A Rumballe, Derina E Sweeney, M Todd Valerius, Gen Yamada, Yiya Yang, Jing Yu A high-resolution anatomical ontology of the developing murine genitourinary tract. Gene Expr. Patterns: 2007, 7(6);680-99 PMID:17452023 | PMC2117077
- ↑ Yutaka Maeda, Vrushank Davé, Jeffrey A Whitsett Transcriptional control of lung morphogenesis. Physiol. Rev.: 2007, 87(1);219-44 PMID:17237346
- ↑ Mathias V Schmidt, M Schmidt, L Enthoven, M van der Mark, S Levine, E R de Kloet, M S Oitzl The postnatal development of the hypothalamic-pituitary-adrenal axis in the mouse. Int. J. Dev. Neurosci.: 2003, 21(3);125-32 PMID:12711350
- ↑ J A Beck, S Lloyd, M Hafezparast, M Lennon-Pierce, J T Eppig, M F Festing, E M Fisher Genealogies of mouse inbred strains. Nat. Genet.: 2000, 24(1);23-5 PMID:10615122
| Parental genome
| Nodal cilia rotation
| Somitogenesis |
|Migration 1||Migration 2||Migration 3||microCT E11.5|
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name.
- The e-Mouse Atlas Project EMAP
Mouse Gene Expression
- EMAGE is a database of in situ gene expression data in the mouse embryo and an accompanying suite of tools to search and analyse the data. (All site content, except where otherwise noted, is licensed under a Creative Commons Attribution License.)
- Eurexpress transcriptome atlas a High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo.
- GenePaint.org is a digital atlas of gene expression patterns in the mouse.
- Mouse Imaging Centre
- NCBI- Mouse Genome Sequencing
- Mouse Genome Informatics
- EBI- Mouse Genome Sequencing
- ANEX Rat and Mouse Database - (University of Tokushima)
- Genetic and Physical Maps of the Mouse Genome (Whitehead Institute/MIT)
- Genetic Location Database (University of Southampton)
- Koop Human and Mouse Genome Project Research (University of Victoria)
- Genome Systems, Inc.
Mouse Jackson Laboratory
- Jackson Laboratory
- Jackson Laboratory Induced Mutant Resource
- Jackson Laboratory BioInformatics Electronic Bulletin Board System
- Mouse Inbred Strains (Jackson Labs)
- Mouse Linkage Map
- Mouse Nomenclature Rules and Guidelines (Jackson Labs)
- Helicobacter hepaticus, a Recently Recognized Bacterial Pathogen, Associated with Chronic Hepatitis and Hepatocellular Neoplasia in Laboratory Mice (CDC)
- Helicobacter Infection in Laboratory Mice: History, Significance, Detection and Management (Charles River)
- Infectious Diseases of Mice and Rats (NAS-ILAR)
- Infectious Diseases of Mice and Rats Companion Guide (NAS-ILAR)
- Infectious Diseases of Mice and Rats (AFIP-POLA)
- Murine Helicobacters (NCI)
- NIH List of Available Knockout Mice | Factsheet
- International Knockout Mouse Consortium IKMC | Research using IKMC products
- NC3Rs Mouse databases
- Mammary Transgene Database (Baylor College of Medicine)
- TBASE - Transgenic/Targeted Mutation Database
- B Universal Ltd.
- BIG BLUE (Big Blue Transgenic Mouse Mailing List Archives)
- Big Blue lacI Transgenic Mouse Page
- Charles River Laboratories, Inc.
- Chrysalis DNX Transgenic Services
Mouse Transgenic Facilities
- Transgenic Animal Facility (University of Connecticut Biotechnology Center)
- Transgenic Animal Facility (University of Illinois Biotechnology Center)
- Transgenic Animal Facility (University of Iowa)
- Transgenic Animal Facility (Karolinska Institute)
- Transgenic Animal Facility (University of Western Australia)
- Transgenic Animal Facility (University of Wisconsin Biotechnology Center)
- Transgenic Animal Model Core (University of Michigan)
- Transgenic Core (Baylor College of Medicine SPORE)
- Transgenic Facility (University of Colorado Health Science Center)
- Transgenic Facility (University of Kentucky)
- Transgenic & Knockout Mouse Facility (University of Maryland at Baltimore)
- Transgenic Models of Skin Disease Core (Harvard Skin Disease Research Center)
- Transgenic Mouse Core Facilities (Mount Sinai School of Medicine)
- Transgenic Mouse Core Facility (University of Virginia)
- Transgenic Mouse Facility (Columbia-Presbyterian Cancer Center)
- Transgenic Mouse Facility (Duke Comprehensive Cancer Center)
- Transgenic Mouse Facility (SUNY-Stony Brook)
- Transgenic Mouse Facility (University of California-Irvine)
- Transgenic Mouse Facility (University of South Carolina)
- GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Renal Development Tutorial | Genital Development Tutorial
Mouse Unsorted Links
- Covance Research Products
- Edison Biotechnology Institute (Ohio University)
- Encyclopedia of the Mouse Genome (Jackson Laboratory)
- EUCIB Mouse Backcross Database (MBx)
- Harlan Sprague Dawley, Inc.
- It's a Knockout
- Jackson Laboratory Gopher Server
- Lane List of Named Mutations and Polymorphic Loci
- Lexicon Genetics, Inc.
- Lymphocytic Choriomeningitis Virus: An Unrecognized Teratogenic Pathogen (CDC)
- M & B Breeding and Research Centre Ltd.
- Map Manager Software
- Mice and Men: Making the Most of Our Similarities (ORNL)
- Microinjection Workshop
- Mouse Atlas and Gene Expression Database Project
- Mouse Cytogenetic Map Image
- Mouse Idiograms
- Mouse Linkage Map
- Mouse and Rat Research Home Page
- MouseUp HyperCard Transgenic Mouse Database (Macintosh)
- MRC Mouse Genome Centre
- National Institute of Genetics Mouse Genetic Resources (Japan)
- NIH Animal Genetic Resource
- NIH Directory of Transgenic Mice
- NIH Mouse Club
- Of Mice and Men (HHMI's Blazing a Genetic Trail)
- Online Mendelian Inheritance in Animals (University of Sydney)
- Pathology of Genetically-altered Mice
- Portable Dictionary of the Mouse Genome
- Review of Pasteurella pneumotropica (Charles River)
- The Mouse in Science: Cancer Research
- The Mouse in Science: Monoclonal Antibodies
- The Mouse in Science: Vaccines
- The Mouse in Science: Why Mice?
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Cite this page: Hill, M.A. (2014) Embryology Mouse Development. Retrieved April 16, 2014, from http://embryology.med.unsw.edu.au/embryology/index.php?title=Mouse_Development
- Dr Mark Hill 2014, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G