Medaka Development
Contents |
Introduction
Medaka Oryzias latipes or Japanese rice fish is a member of the killifish family first described in 1846 and has been widely used as a aquarium fish. A modified aquarium version with a genetically modified fluorescent (GFP) version also now available in some countries.
A recent study by Iwamatsu T., 2004[1] has characterised the stages of normal fish development.
Medaka fish were also the first for the first vertebrate animal to mate in space (The International Microgravity Laboratory IML-2/STS-65 mission in 1994) as a developmental model for space experiments. The fish has also been used in studies of pigmentation development.
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Some Recent Findings
- Osterix-mCherry transgenic medaka for in vivo imaging of bone formation[2]
- Induction of otic structures by canonical Wnt signalling in medaka.[3]
Taxon
cellular organisms; Eukaryota; Fungi/Metazoa group; Metazoa; Eumetazoa; Bilateria; Coelomata; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Elopocephala; Clupeocephala; Euteleostei; Neognathi; Neoteleostei; Eurypterygii; Ctenosquamata; Acanthomorpha; Euacanthomorpha; Holacanthopterygii; Acanthopterygii; Euacanthopterygii; Percomorpha; Smegmamorpha; Atherinomorpha; Beloniformes; Adrianichthyoidei; Adrianichthyidae; Oryziinae; Oryzias
Development Overview
Development has been characterised by light microscope observation into 39 prehatch stages and 6 posthatch stages.[1]
Prehatch features observed included: number and size of blastomeres, form of the blastoderm, extent of epiboly, central nervous system, number and form of somites, optic and otic, notochord, heart, blood circulation, the size and movement of the body, tail, membranous fin (fin fold), viscera (liver gallbladder, gut tube), spleen and swim (air) bladder.
Posthatch features observed included: fins, scales and secondary sexual characteristics.
Developmental Stages
| Stage | Time | Event |
| 0 | Unfertilized eggs | |
| 1 | 3 min | Activated egg |
| 2 | Blastodisc | |
| 3 | 1 h 5 min | 2 cell |
| 4 | 1 h 45 min | 4 cell |
| 5 | 2 h 20 min | 8 cell |
| 6 | 2 h 55 min | 16 cell |
| 7 | 3 h 30 min | 32 cell |
| 8 | 4 h 5 min | Early morula |
| 9 | 5 h 15 min | Late morula |
| 10 | 6 h 30 min | Early blastula |
| 11 | 8 h 15 min | Late blastula |
| 12 | 10 h 20 min | Pre-early gastrula |
| 13 | 13 h | Early gastrula |
| 14 | 15 h | Pre-mid-gastrula |
| 15 | 17 h 30 min | Mid-gastrula |
| 16 | 21 h | Late gastrula |
| 17 | 1 day 1 h | Early neurula (head formation) |
| 18 | 1 day 2 h | Late neurula (optic bud formation) |
| 19 | 1 day 3 h 30 min | 2 somite |
| 20 | 1 day 7 h 30 min | 4 somite |
| 21 | 1 day 10 h | 6 somite (brain regionalization and otic vesicle formation) |
| 22 | 1 day 14 h | 9 somite (appearance of heart anlage) |
| 23 | 1 day 17 h | 12 somite (formation of tubular heart) |
| 24 | 1 day 20 h | 16 somite (start of heart beating) |
| 25 | 2 days 2 h | 18–19 somite (onset of blood circulation) |
| 26 | 2 days 6 h | 22 somite (development of guanophores and vacuolization of the notochord) |
| 27 | 2 days 10 h | 24 somite (appearance of pectoral fin bud) |
| 28 | 2 days 16 h | 30 somite (onset of retinal pigmentation) |
| 29 | 3 days 2 h | 34 somite (internal ear formation) |
| 30 | 3 days 10 h | 35 somite (blood vessel development) |
| 31 | 3 days 23 h | Gill blood vessel formation |
| 32 | 4 days 5 h | Somite completion (formation of pronephros and air bladder) |
| 33 | 4 days 10 h | at which notochord vacuolization is completed |
| 34 | 5 days 1 h | Pectoral fin blood circulation |
| 35 | 5 days 12 h | at which visceral blood vessels form |
| 36 | 6 days | Heart development |
| 37 | 7 days | Pericardial cavity formation |
| 38 | 8 days | Spleen development (differentiation of caudal fin begins) |
| 39 | 9 days | Hatching |
| 40 | 1st fry | |
| 41 | ||
| 42 | ||
| 43 | ||
| 44 | ||
| 45 |
National BioResource Project Medaka
The National BioResource Project Medaka (NBRP Medaka): an integrated bioresource for biological and biomedical sciences[4]"The Japanese government has supported the development of Medaka Bioresources since 2002. The second term of the Medaka Bioresource Project started in 2007. The National Institute for Basic Biology and Niigata University were selected as the core organizations for this project. More than 400 strains including more than 300 spontaneous and induced mutants, 8 inbred lines, 21 transgenic lines, 20 medaka-related species and 66 wild stock lines of medaka are now being provided to the scientific community and educational non-profit organizations. In addition to these live fish, NBRP Medaka is also able to provide cDNA/EST clones such as full-length cDNA and BAC/fosmid clones covering 90% of the medaka genome."
References
- ↑ 1.0 1.1 Takashi Iwamatsu Stages of normal development in the medaka Oryzias latipes. Mech. Dev.: 2004, 121(7-8);605-18 PMID:15210170
- ↑ Joerg Renn, Christoph Winkler Osterix-mCherry transgenic medaka for in vivo imaging of bone formation. Dev. Dyn.: 2009, 238(1);241-8 PMID:19097055
- ↑ Baubak Bajoghli, Narges Aghaallaei, Gerlinde Jung, Thomas Czerny Induction of otic structures by canonical Wnt signalling in medaka. Dev. Genes Evol.: 2009, 219(8);391-8 PMID:19760182
- ↑ Takao Sasado, Minoru Tanaka, Kayo Kobayashi, Tadashi Sato, Mitsuru Sakaizumi, Kiyoshi Naruse The National BioResource Project Medaka (NBRP Medaka): an integrated bioresource for biological and biomedical sciences. Exp. Anim.: 2010, 59(1);13-23 PMID:20224166
Articles
Daniel Liedtke, Isabell Erhard, Manfred Schartl snail gene expression in the medaka, Oryzias latipes. Gene Expr. Patterns: , 11(3-4);181-9 PMID:21094700
Akihiro Shima, Hiroshi Mitani Medaka as a research organism: past, present and future. Mech. Dev.: 2004, 121(7-8);599-604 PMID:15210169
Search Pubmed
Search Pubmed: Medaka Development
External Links
- National BioResource Project Medaka (NBRP Medaka) http://shigen.lab.nig.ac.jp/medaka/
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Cite this page:
Hill, M.A. (2013) Medaka Development. Retrieved June 19, 2013, from http://php.med.unsw.edu.au/embryology/index.php?title=Medaka_Development
- Dr Mark Hill 2013, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
