File:Renal - podocyte development 01.jpg

Renal - Mouse Podocyte Development

MafB-GFP mice show restricted expression of GFP in podocytes.

Even as early as E13.5 in the S-shaped bodies the prospective podocytes were uniquely labeled by GFP (Fig. 1, left panels). At this stage of development the immature podocytes form a single layer of cells adjacent to the glomerular cleft. As development progresses a capillary loop forms within the cleft and the early glomerulus is encircled by podocytes (Fig. 1, right panels).

Cryostat sections of E15.5 MafB-GFP transgenic kidneys with visible (above panels) and fluorescent (below) illumination. An S-shaped body and glomerulus are marked in above panels (arrows), and prospective podocytes in these structures are shown to be GFP positive in lower panels.

Links: Renal System Development | Image - Renal - podocyte development | Image - S-shaped body stage | Image - early glomerulus

Reference

Brunskill EW, Georgas K, Rumballe B, Little MH, Potter SS (2011) Defining the Molecular Character of the Developing and Adult Kidney Podocyte. PLoS ONE 6(9): e24640. doi:10.1371/journal.pone.0024640

PLoS One.

© 2011 Brunskill et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Figure 1. Journal.pone.0024640.g001.png doi:10.1371/journal.pone.0024640.g001