File:Chicken- wing cartilage.jpg

Alcian-blue staining of the cartilaginous skeleton of embryonic chicken wings

A - C Control wings.

A1 - C1 Wings after injection of Wnt6-CHO cells at HH-stage 20-21 and 4 days reincubation.

• Skeletal elements are shorter in length compared to the control wings.
• A1 to C1 illustrate increasingly severe defects depending on the quantity and spreading of injected cells
  • A1 being the mildest - only the humerus is affected.
  • B1 stylopod and zeugopod are affected.
  • C1 being the severest phenotype - the entire limb is affected.

Scale bar 500 μm.

"Our results suggest that the inhibitory influence of the ectoderm on limb chondrogenesis acts on an early stage of chondrogenesis upsteam of Sox9 and Collagen II. We identify Wnt6 as a candidate mediator of ectodermal chondrogenic inhibition in vivo. We propose a model of Wnt-mediated centripetal patterning of the limb by the surface ectoderm."

Links: Chicken Development | Wnt | Limb Development | Cartilage Development

Original File name: Figure 2. 1471-213X-10-32-2-l.jpg http://www.biomedcentral.com/1471-213X/10/32/figure/F2

Reference

<pubmed>20334703</pubmed>| PMC2859743 | BMC Dev. Biol.

Geetha-Loganathan et al. BMC Developmental Biology 2010 10:32 doi:10.1186/1471-213X-10-32

© 2010 Geetha-Loganathan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.