Our adult skeleton forms from a larger number of developmental elements that are replaced and fuse. In development there are 2 separate signaling pathways for pattern formation and the formation of bone itself. Furthermore bone formation can be divided into 2 specific forms that occur in anatomically different regions. This practical class will describe the development and structure of bone and finish with a study of abnormalities associated with bone.
The image shown to the left shows a histological section through the developing lower limb at the level of a developing joint (knee), surrounding the developing bone is cartilage, skeletal muscles and connective tissue of the limb.
- Musculoskeletal Links: Introduction | Mesoderm | Somitogenesis | Limb | Cartilage | Bone | Bone Timeline | Axial Skeleton | Skull | Joint | Muscle | Tendon | Diaphragm | Lecture - Musculoskeletal Development | Abnormalities | Limb Abnormalities | Cartilage Histology | Bone Histology | Skeletal Muscle Histology | Category:Musculoskeletal
Chondroblasts and Chondrocytes
Immature and mature cartilage forming cells located at articular cartilage regions.
- Occurs mainly in immature cartilage.
- Chondroblasts in existing cartilage divide and form small groups of cells (isogenous groups) which produce matrix to become separated from each other by a thin partition of matrix.
- Occurs also in mature cartilage.
- Mesenchymal cells surrounding the cartilage in the deep part of the perichondrium (or the chondrogenic layer) differentiate into chondroblasts.
- Bone Histology: Cartilage Histology | Histology Stains | Histology | Cartilage Development | Bone Development
Human Fetal Head (12 week)
- an anthraquinone derivative used to identify calcium in tissue sections
- calcium forms an Alizarin Red S-calcium complex in a chelation process and the end product is also birefringent.
- reaction can also identify magnesium, manganese, barium, strontium, and iron may interfere
- these elements usually in too low concentration to interfere with the staining
- acronym for hematoxylin and eosin stain
- hematoxylin - basic dye which colors basophilic structures with blue-purple hue (nucleus, DNA, RNA)
- eosin Y - acidic alcohol-based which colors eosinophilic structures bright pink (cytoplasm, extracellular matrix, protein)
- Van Gieson's Stain is a mixture of picric acid and acid fuchsin used for differential staining of collagen and other connective tissue.
- Nuclei - stains brownish black to black
- Collagen (fibrous connective tissue) - stains pink or deep red
- Muscle, Cytoplasm, RBC and Fibrin - stains yellow
- Links: Histology Stains
- Anatomy of the Human Body (H. Gray, 1918.) historical anatomy text Osteology
- Molecular Biology of the Cell Bone Is Continually Remodeled by the Cells Within It | Image: Figure 22-52. Deposition of bone matrix by osteoblasts | Image: Figure 22-56. The development of a long bone
- Molecular Cell Biology Mutations in Collagen Reveal Aspects of Its Structure and Biosynthesis
- The Cell- A Molecular Approach Steroid Hormones and the Steroid Receptor Superfamily
- Clinical Methods: The History, Physical, and Laboratory Examinations 100. Alkaline Phosphatase and Gamma Glutamyltransferase
- Endocrinology: An Integrated Approach by Nussey, S.S. and Whitehead, S.A. Endocrinology: Definition and causes of osteoporosis
- Developmental Biology 6th ed. by Gilbert, Scott F. Figure 14.13. Schematic diagram of endochondral ossification | Aging: The Biology of Senescence
- Pubmed ossification
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name.
- Virtual Slidebox of Histology (USA) Skeletal system
- e-radiography Ossification
- UWA Blue Histology Histology - Cartilage
- haematopoiesis (Greek, haima = "blood"; poiesis = "to make") the process of blood cell formation.
- Howship's lacuna - (resorptive bay) the historic name for the shallow bay or cavity lying directly under an osteoclast. This is the site of bone matrix resorption.
- lacuna - (Latin, lacuna = “ditch, gap” diminutive form of lacus = “lake”) lacunae is the plural, cavity in bone or cartilage for cell.
- lamellar bone - the highly organized strong bone matrix deposited in concentric sheets with a low proportion of osteocytes. Many collagen fibers parallel to each other in the same layer.
- osteon - (Haversian system) the functional unit of compact bone. Consists of a central canal (Haversian canal) surrounded by lamellar bone matrix within which osteocytes reside.
- resorptive bay - (Howship's lacuna) the shallow bay or cavity lying directly under an osteoclast. This is the site of bone matrix resorption.
- suture - in the skull a form of articulation where the contiguous margins of the bones are united by a thin layer of fibrous tissue.
- woven bone - the first deposited weaker bone matrix with many osteocytes and a matrix disorganized structure. Replaced by lamellar bone. Seen in developing, healing and bone disease.
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Cite this page:
Hill, M.A. (2013) Cartilage Histology. Retrieved June 20, 2013, from http://php.med.unsw.edu.au/embryology/index.php?title=Cartilage_Histology
- Dr Mark Hill 2013, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G