File:Interphase ER tomography.jpg

Figure 2. Electron tomographic analysis of the interphase ER from two different cell depths. (A and B) Three successive 200- or 250-nm thick sections from two ssHRP-KDEL/CHO-K1 cells were subjected to ET. The sections were the first ones from the bottom of the cell next to the coverslip (A) or from the middle of the cell depth (B) and show long interconnected tubules in the cell periphery and sheets in the more central parts of the cell (illustration demonstrates the cell depth where sections for imaging were selected). In A, (b) is a side view of the model in (a), and reveals the flat area of the lamellipodia. The ER is modeled in yellow and the NE in red. In B, the Golgi area is highlighted with darker gray. Bars, 2 μm. (C) Models of the different types of branch points between two sheets (a and Video 1), a sheet and a tubule (b), or two tubules (c). Bars, 500 nm.

J Cell Biol. 2007 December 3; 179(5): 895–909. doi: 10.1083/jcb.200705112. Copyright © 2007, The Rockefeller University Press