File:Exocytic transport.mov

Figure 3 Exocytic transport intermediates carrying GPI-GFP.

GPI-GFP was expressed in COS-7 cells. All fluorescence outside of the region indicated in the prebleach image was eliminated by photobleaching. Note that the fluorescence intensity of the Golgi region is saturated (pixel value equals 255 for 8-bit images) in all of these images to allow visualization of the less bright transport intermediates. Times in subsequent panels are relative to the end of the photobleach. Supplemental video available at http://www.jcb.org/cgi/content/full/153/3/529/DC1. Bar, 5 µm.

Original File name: JCB0010136.V3B

Video 3B (9.7MB) http://jcb.rupress.org/cgi/content/full/153/3/529/F3/DC2

http://jcb.rupress.org/cgi/content/full/153/3/529#F3

Rapid cycling of lipid raft markers between the cell surface and Golgi complex. Nichols BJ, Kenworthy AK, Polishchuk RS, Lodge R, Roberts TH, Hirschberg K, Phair RD, Lippincott-Schwartz J. J Cell Biol. 2001 Apr 30;153(3):529-41. PMID: 11331304

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