Figure 3 Exocytic transport intermediates carrying GPI-GFP.
GPI-GFP was expressed in COS-7 cells. All fluorescence outside of the region indicated in the prebleach image was eliminated by photobleaching. Note that the fluorescence intensity of the Golgi region is saturated (pixel value equals 255 for 8-bit images) in all of these images to allow visualization of the less bright transport intermediates. Times in subsequent panels are relative to the end of the photobleach. Supplemental video available at http://www.jcb.org/cgi/content/full/153/3/529/DC1. Bar, 5 µm.
Original File name: JCB0010136.V3B
Video 3B (9.7MB) http://jcb.rupress.org/cgi/content/full/153/3/529/F3/DC2
Rapid cycling of lipid raft markers between the cell surface and Golgi complex. Nichols BJ, Kenworthy AK, Polishchuk RS, Lodge R, Roberts TH, Hirschberg K, Phair RD, Lippincott-Schwartz J. J Cell Biol. 2001 Apr 30;153(3):529-41. PMID: 11331304
© 2001 Nichols et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Click on a date/time to view the file as it appeared at that time.
|current||17:42, 17 March 2010||(2.99 MB)||S8600021||Figure 3 Exocytic transport intermediates carrying GPI-GFP. GPI-GFP was expressed in COS-7 cells. All fluorescence outside of the region indicated in the prebleach image was eliminated by photobleaching. Note that the fluorescence intensity of the Golgi|
- You cannot overwrite this file.
- Edit this file using an external application (See the setup instructions for more information)